Difference between revisions of "Part:BBa K2100012:Experience"

 
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===Applications of BBa_K2100012===
 
===Applications of BBa_K2100012===
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The MIT iGEM team used the hEF1a promoter to constitutively express fluorescent proteins as transfection markers in our experiments in HEK293, tHESC, MCF-7, and ISH cells. The amount of fluorescent units observed is an indication of the number of plasmids a particular cell uptakes. The graph below shows two single color controls from one of our experiments. We transfected one well of HEK293 cells with hEF1a-mKate and another with hEF1a-eYFP. The cells that uptook the hEF1a-mKate plasmids showed an increase in only the red fluorescent output, while the cells that uptook the hEF1a-eYFP plasmids showed a clear increase in only the yellow fluorescent output.
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https://static.igem.org/mediawiki/parts/c/c8/T--MIT--hEF1a.png
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Here is an image of tHESC cells constitutively expressing mKate florescence under the hEF1a promoter.
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https://static.igem.org/mediawiki/parts/9/92/T--MIT--khb_thesc_red.jpeg
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 22:07, 22 October 2016


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Applications of BBa_K2100012

The MIT iGEM team used the hEF1a promoter to constitutively express fluorescent proteins as transfection markers in our experiments in HEK293, tHESC, MCF-7, and ISH cells. The amount of fluorescent units observed is an indication of the number of plasmids a particular cell uptakes. The graph below shows two single color controls from one of our experiments. We transfected one well of HEK293 cells with hEF1a-mKate and another with hEF1a-eYFP. The cells that uptook the hEF1a-mKate plasmids showed an increase in only the red fluorescent output, while the cells that uptook the hEF1a-eYFP plasmids showed a clear increase in only the yellow fluorescent output.

T--MIT--hEF1a.png

Here is an image of tHESC cells constitutively expressing mKate florescence under the hEF1a promoter.

T--MIT--khb_thesc_red.jpeg

User Reviews

UNIQ586c0bec819f37ea-partinfo-00000000-QINU UNIQ586c0bec819f37ea-partinfo-00000001-QINU