Difference between revisions of "Part:BBa K1963010"

Revision as of 22:06, 22 October 2016

An sRNA system for down-regulation of E. coli fliC encoding flagellin

This is a multi-sequence system for small RNA (sRNA) production in an E. coli host - or especially with co-expression of E. coli Hfq BBa_K1963000. The system is based on the BBa_K1963002 template and has the proD strong promoter, followed by a short antisense sequence targeting the RBS and 5' end of the fliC transcript, followed by the E. coli micC hairpin, followed by the strong terminator sequence T1/TE. The anti fliC sRNA is placed after C-193. The system should impair motility of E. coli.

Usage and Biology

Sequence and Features