Difference between revisions of "Part:BBa K1893007"

 
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<partinfo>BBa_K1893007 short</partinfo>
 
<partinfo>BBa_K1893007 short</partinfo>
  
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Stuff
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The gene encoding the transcriptional activator CinR is downstream a constitutive Anderson promoter, j23101. CinR is activated by 3O-C14-HSL. Included in the construct is the CinR-activated promoter pCin upstream of a reporter sequence including GFP. This part was designed so that we could characterize the CinR quorum sensing system and determine activation ranges and crosstalk data.
  
 
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Revision as of 19:46, 22 October 2016


Cin receiver with GFP (CinR+pCin+GFP)

The gene encoding the transcriptional activator CinR is downstream a constitutive Anderson promoter, j23101. CinR is activated by 3O-C14-HSL. Included in the construct is the CinR-activated promoter pCin upstream of a reporter sequence including GFP. This part was designed so that we could characterize the CinR quorum sensing system and determine activation ranges and crosstalk data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1698