Difference between revisions of "Part:BBa K1893003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the | + | This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for C4-HSL-induced expression of genes inserted downstream of the pRhl promoter, which is, in this case, GFPmut3b. GFPmut3b was included so we could characterise the activation range of the Rhl quorum sensing system. |
Latest revision as of 19:19, 22 October 2016
Rhl receiver with GFP reporter (RhlR+pRhl+GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776
Illegal BsaI.rc site found at 1658
Design Notes
This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for C4-HSL-induced expression of genes inserted downstream of the pRhl promoter, which is, in this case, GFPmut3b. GFPmut3b was included so we could characterise the activation range of the Rhl quorum sensing system.
Source
Freemont Lab, Imperial College London