Difference between revisions of "Part:BBa K1893000:Design"
 
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<partinfo>BBa_K1893000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1893000 SequenceAndFeatures</partinfo>
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tttacagctagctcagtcctaggtattatgctagcTACTAGAGaaagaggagaaaTACTAGatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataaTACTAGAGccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataTACTAGAGcctttccgaaacgaaacaagttggattttgcacctaccagaactggtagttctgacctgtggctatcttcgaaggcatcgatattatgcacattggaactcttcatgacataacgccgag
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===Design Notes===
 
===Design Notes===
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This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for  3O-C12-HSL-induced expression of genes inserted downstream of the pLas promoter.
  
  
  
 
===Source===
 
===Source===
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Freemont Lab, Imperial College London
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===References===
 
===References===

Latest revision as of 19:18, 22 October 2016


Las receiver (LasR+pLas)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for 3O-C12-HSL-induced expression of genes inserted downstream of the pLas promoter.


Source

Freemont Lab, Imperial College London

References