Difference between revisions of "Part:BBa K1893003:Design"
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===Design Notes===
 
===Design Notes===
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This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pRhl promoter.
+
This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pRhl promoter, which is, in this case, GFPmut3b.  
  
  

Revision as of 19:14, 22 October 2016


Rhl receiver with GFP reporter (RhlR+pRhl+GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 1658


Design Notes

This part does not include an LVA tag on the RhlR gene. This means that the protein is not rapidly degraded. When active, the RhlR protein binds to the pRhl promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pRhl promoter, which is, in this case, GFPmut3b.


Source

Freemont Lab, Imperial College London

References