Difference between revisions of "Part:BBa K1962012"
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This is a composite part that is designed to induce production of the Immunity Protein to Colicin E3 in the presence of bile. The composite part consists of the bile salt responsive promoter (<partinfo>BBa_K318514</partinfo>), RBS (<partinfo>BBa_B0030</partinfo>) and the Colicin E3 Immunity protein (<partinfo>BBa_K1962004</partinfo>). For best results, use in conjunction with the transcriptional activator RamA (<partinfo>BBa_K1962009</partinfo>). | This is a composite part that is designed to induce production of the Immunity Protein to Colicin E3 in the presence of bile. The composite part consists of the bile salt responsive promoter (<partinfo>BBa_K318514</partinfo>), RBS (<partinfo>BBa_B0030</partinfo>) and the Colicin E3 Immunity protein (<partinfo>BBa_K1962004</partinfo>). For best results, use in conjunction with the transcriptional activator RamA (<partinfo>BBa_K1962009</partinfo>). | ||
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| + | https://static.igem.org/mediawiki/2016/9/90/T--Dundee--Results17.png | ||
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Revision as of 18:54, 22 October 2016
A device for expression of Im-E3 in response to bile salts
This is a composite part that is designed to induce production of the Immunity Protein to Colicin E3 in the presence of bile. The composite part consists of the bile salt responsive promoter (BBa_K318514), RBS (BBa_B0030) and the Colicin E3 Immunity protein (BBa_K1962004). For best results, use in conjunction with the transcriptional activator RamA (BBa_K1962009).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 471
Illegal XhoI site found at 785 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]