Difference between revisions of "Part:BBa K1962010"
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This is a composite part which consists of the bile salt responsive promoter (<partinfo>BBa_K318514</partinfo>) and a  combined ribosome binding site / GFP / terminators biobrick (<partinfo>BBa_E0840</partinfo>). The <i>acrRA</i> operon is found in <i>Salmonella enterica</i> strain LT2 and the promoter sequence contains a RamA (<partinfo>BBa_K1962009</partinfo>) binding sequence.
 
This is a composite part which consists of the bile salt responsive promoter (<partinfo>BBa_K318514</partinfo>) and a  combined ribosome binding site / GFP / terminators biobrick (<partinfo>BBa_E0840</partinfo>). The <i>acrRA</i> operon is found in <i>Salmonella enterica</i> strain LT2 and the promoter sequence contains a RamA (<partinfo>BBa_K1962009</partinfo>) binding sequence.
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Usage and Biology
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https://static.igem.org/mediawiki/2016/1/1b/T--Dundee--GreenResults2.png
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https://static.igem.org/mediawiki/2016/2/2b/File-T--Dundee--Resultsfrulhuq18.png
  
  
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===Usage and Biology===
 
  
 
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Revision as of 18:53, 22 October 2016


Bile Salts Sensing Device

This is a composite part which consists of the bile salt responsive promoter (BBa_K318514) and a combined ribosome binding site / GFP / terminators biobrick (BBa_E0840). The acrRA operon is found in Salmonella enterica strain LT2 and the promoter sequence contains a RamA (BBa_K1962009) binding sequence.

Usage and Biology

T--Dundee--GreenResults2.png

File-T--Dundee--Resultsfrulhuq18.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal XhoI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1508