Difference between revisions of "Part:BBa K2074034"
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we use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
 
we use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
−
[[File:T--FAFU-CHINA--4+E.png|600px|thumb|center|'''Figure.1''' The gel electrophoresis result of Cry11Aa+FMDV 2A+EGFP]]
+
[[File:Confocalcry11.png|600px|thumb|center|'''Figure.1''']]
 +
 
 +
[[File:T--FAFU-CHINA--4+E.png|600px|thumb|center|'''Figure.2''' The gel electrophoresis result of Cry11Aa+FMDV 2A+EGFP]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:47, 22 October 2016


Cry11Aa(Codon optimization)+2A+EGFP(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

we use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

Figure.1
Figure.2 The gel electrophoresis result of Cry11Aa+FMDV 2A+EGFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
    Illegal NgoMIV site found at 2740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440