Difference between revisions of "Part:BBa K2120307"

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__NOTOC__
 
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<partinfo>BBa_K2120307 short</partinfo>
 
<partinfo>BBa_K2120307 short</partinfo>
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pBAD is a promoter induced by arabinose and arac is the repressor. We use it to test the expression of reporter gene in our validation circuit .And tetR is an inhibitor which is built to repress the expression of Ptet promoter. We use it to control the “on” or ”off” state of toxin gene. In order to avoid leaking out, We reserve  【BBa_K2120303 】.  
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We reserve K2120303([https://parts.igem.org/Part:BBa_K2120303 BBa_K2120303]) and ligate B0015 at the end of ''araC''. This device is designed to construct K2120311([https://parts.igem.org/Part:BBa_K2120311 BBa_K2120311]).  
  
 
    
 
    

Latest revision as of 17:17, 22 October 2016


B0015+tetR+B0034+pBAD-AraC+B0015

We reserve K2120303(BBa_K2120303) and ligate B0015 at the end of araC. This device is designed to construct K2120311(BBa_K2120311).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 911
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1076
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1093