Difference between revisions of "Part:BBa K1983015"

 
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<partinfo>BBa_K1983015 short</partinfo>
 
<partinfo>BBa_K1983015 short</partinfo>
  
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BBa_K1983015
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<h2>Overview</h2>
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This biobrick part contains two coding elements:
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<b>([https://parts.igem.org/Part:BBa_K1983014 BBa_K1983014])</b>: PheP, which is labeled with N-6XHis-Tag and put under a constitutive promoter ([https://parts.igem.org/Part:BBa_K823005 BBa_K823005]), high strength RBS 9[https://parts.igem.org/Part:BBa_J61132 BBa_J61132]) and terminator ([https://parts.igem.org/Part:BBa_B0017 BBa_B0017]).
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A basic biobrick part <b>([https://parts.igem.org/Part:BBa_K1983011 BBa_K1983011])</b>: tRNA-Phe (pheU) under constitutive promoter and terminatorPheP (Phenylalanine-specific permease) is a natural phenylalanine membrane transporter of the bacterium Escherichia coli.
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PheP is a single integral membrane transporter which selectively transports L-phenylalanine and L-tyrosine as an antiporter using proton motive force. The activity of this transporter under natural expression is known to be 9 and 17,5 nmol/mgDW(cells) for L-phenylalanine and L-tyrosine respectively [1].
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pheU gene from Escherichia coli encodes a tRNA transporting L-phenylalanine and is under an upstream constitutive promoter and a terminator found after the tRNA.
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Revision as of 12:26, 22 October 2016


PheP and tRNA-Phe under constitutive promoters

Overview

This biobrick part contains two coding elements: (BBa_K1983014): PheP, which is labeled with N-6XHis-Tag and put under a constitutive promoter (BBa_K823005), high strength RBS 9BBa_J61132) and terminator (BBa_B0017). A basic biobrick part (BBa_K1983011): tRNA-Phe (pheU) under constitutive promoter and terminatorPheP (Phenylalanine-specific permease) is a natural phenylalanine membrane transporter of the bacterium Escherichia coli.

PheP is a single integral membrane transporter which selectively transports L-phenylalanine and L-tyrosine as an antiporter using proton motive force. The activity of this transporter under natural expression is known to be 9 and 17,5 nmol/mgDW(cells) for L-phenylalanine and L-tyrosine respectively [1].

pheU gene from Escherichia coli encodes a tRNA transporting L-phenylalanine and is under an upstream constitutive promoter and a terminator found after the tRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 98
    Illegal NheI site found at 1669
    Illegal NheI site found at 1692
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]