Difference between revisions of "Part:BBa K1954002:Design"
(Replaced content with " __NOTOC__ <partinfo>BBa_K1954002 short</partinfo> <partinfo>BBa_K1954002 SequenceAndFeatures</partinfo> ===Design Notes=== Mutagenesis of UCL iGEM 2009 BBa_K239009. T...")
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The following is the sequence of Vitellogenin proposal sequence:
 
The following is the sequence of Vitellogenin proposal sequence:
−
 
−
gaattcgcggccgcttctagagagatcttttttgtagaaatgtcttggtgtcctcgtccaatcaggtagccatctctgaaatatctggctccgttgcaactccgaacgacctgctggcaacgtaaaattctccggggtaaaacttaaatgtggagtaatggaaccagaaacgtctcttcccttctctctccttccaccgcccgttaccgtccctaggaaattttactctgctggagagcttcttctacggcccccttgcagcaatgctcttcccagcattacgttgcgggtaaaacggaggtcgtgtacccgacctagcagcccagggatggaaaagtcccggccgtcgctggcaataatagcgggcggacgcatgtcatgagattattggaaaccaccagaatcgaatataaaaggcgaacacctttcccaattttggtttctcctgacccaaagactttaaatttaatttatttgtccctatttcaatcaattgaacaactaatacaaatgttggccccaggattcgcctctgttaagacctacatgtacaaatatgaagcggttcttatgggcggcctgcctgaagagggcctggctcgagctggggttaaaatccggggcaaagttttgatcagtgcaacaagtgccaacgactacattctgaagcttgtagaccctcagttgctggagtacagtggcatctggcccaaagatcctttccatccagccaccaagctcaccacagccctggctactcagctctcgacaccgatcaagtttgagtatacaaacggcgttgtcgggagactggctgcacctcctggggtctccacaacagtgctgaatatctacaggggaatcatcaacctcctgcaactgaatgtaaagaagacacagaacgtctacgagatgcaagagtctggagctcatggtgtgtgcaagaccaactatgtgatcagggaggacgcgagagccgaacgcattcatctgaccaagaccaaggacctgaaccactgccaggagaaaatcatgaaggccatcggcttggaacacgtagagaaatgccatgattgtgaagctagaggaaagagcctgaagggaactgcttcctataactacatcatgaagccagcacccagtggttctctgattatggaggctgtcgctagagaggtcatcgagttctcacctttcaacattttgaatggcgctgctcagatggagtctaagcaaattctgaccttcctggatatcgagaacacccctgtggatcatgccagatacacctatgttcaccgcggatccctgcaatatgagcatggcagcgagattctccagacacccatccatcttctgagggtcacccatgccgaggctcagattgtcagcactctgaaccacctggtagcctccaacgtggccaaggtccatgaagatgcccctctgaagtttgttgagctcatccaggtgatgcgtgtggccagatttgagactattgagtccctctgggctcagtttaaatccagacctgatcacaggtactggttactgaatgctgtcccccacattcgcactcacgctgcgcttaagttcctcattgagaagctccttgctaatgagttaagtgagactgaagctgctatggctctcttggaatgtctgcactctgtgacagctgaccagaaaaccattgaacttgtcagaagcctggctgagaaccacagagtgaaacgtaacgctgtgctcaacgagattgtgatgctgggctggggcactgtaatttccaggttctgtaaagcgcagccatcttgctcatctgatcttgtgacacctgtacatagacaagttgcagaggctgttgaaactggtgacatcgatcagctcactgtcacgctcaaatgcctggataacgctggacatcctgctagcattaagacaatcatgaagttcctgcctggctttggcagtgctgctgcccgagtcccactcaaagttcaggttgacgctgttctagccctgaggagaattgcaaagagggaacccaagatggtccaggaaatagctgctcagttgctcatggaaaagcatctccatgcagaactgcgtatggttgctgccatggtgctctttgagactaaactccccgtgggtctagcagctagcatttccacagccttgatcaaagaaaagaacctgcaagtcgttagctttgtctactcttacatgaaggccatggccaagaccacatcccctgaccacgtttctgttgctgccgcatgtaatgttgccttgaggttcctcaaccccaaattaggcagactgaacttccgctacagccgagccttccatgtggatacctataacaatgcctggatgatgggtgctgccgccagtgccgtcttaattaacgacgctgcaaccgtgttaccaagaatgattatggccaaagcccgtacttacatggccggagcttatgttgatgcttttgaggttggagtgaggactgagggaatccaggaggctcttttgaaaagacgacatgaaaattctgagaatgcagacaggatcaccaagattaaacaagccatgagagctctttctgagtggagggctaatccttcgagccaggccctggcctctatgtatgtgaaggtcttcggacaagaaattgcatttgccaacattgacaaatccaaggttgaccagcttatccagtttgccagtggacctttgagaaacgtattcagagatgctgtgaactctgtgctgtctggttatgcaacacattttgctaaaccaatgctgctcggtgagctccgtctcatccttcccaccactgttgggttgcccatggagatcagcctcattacatccgctgtgactgctgcatctgttgacgtccaagccactgtgtcaccacctctgcctgtcaactaccgagtttcccagcttctggagtccgatatccaactgagggctacagttgctccaagtcttgccatgcagacctatgcattcatgggtgtgaacaccgccttaatccaggctgccgtgatgacaaaagccaaagtttacacagctgttcctgcacagataaaagcaaggattgacattgttaagggcaacttgaaggttgagttcctgtctctccagggcattaacacaattgcatctgcacatgcggagacggttgccattgcaagaaatgtggaagacctcccagccgcaagaagcacaccactgatctcatctgaaactgcatcacaactttcaaaggcctctctcaactcaaagatctccaggatggcatcctctgtgactggtggcatgtctgcgtcatctgaaatcattcctgctgacctgccaagtaagattgggaggaaaatgaaactccctaaaacctacaggaagaaaatccgtgcttcaagcagaatgctaggattcaaggcctacgctgagattgaatctcacaatgccgcctacatcagagactgccctctctacgctctgatcggaaagcatgctgcttctgttaggattgctccagcttctggaccagtcattgagaagattgaagttgagattcaggtcggagataaagcagcagaaaatatgattaaagcgattgacatgagcgaagaggaggaagctcttgaggataagaatgtcctcttgaaaatcaagaaaatactggcacctggtctcaagaacaccacatcatcttcctccagctcctccagctcctcttcatccagctctagctccaacaagtcttcttcatccagttcccgctccagcagctcccagtcatccagctctcgttcccataggtctcgctccagaaagtcccagtctagcagctctcagtcaagccgctctccctcaagctcttcctcctcttcctcctcttcatcatccagatcttcttccaggtcatcttccagatcatcttccagatcttcttctaggtcctcctctcgctccagaactaagatggctgacattgttgctcctattatcacgacgtccaccagagtgagcagttcctccagtcgatcagcctctaacagctcctccagcagtgcttcatacttgctcagctcatctaaaagaagaagcagaagcagaagcagcagcagcagtagcagcagcagcagcagcagcagcagtagcagcagcagcagcagcagcagtagcaagaacagcaagcgcagcaagagcagcaacagcaagtcatcaagctctaggtcctctcggcgcagtgctcagtctaagcaacaactgcttgccttgaagttcagaaagaaccacgtccacaggcatgccatctccacacagcgcggcagcagtcacagcagtgcccgcagcttcgattccatctacaataaggccaagtacctcgctaacacactcactcctgccatgtccattgcaatccgtgccgtgagagtcgaccacaaggtccagggataccagctagcagcttacctggacaaacagaccaatagactgcaattgatttttgccagagtcgctgagaaggacaactggagaatctgtgccgacattgtgcagctgagttcgcacaagctgatggccaagactgcctggggtgctgaatgcaagcaatactccaccatgattgtagctgaaactggtcttttgggtcatgagcccgcagcccgcttgaagctgacctgggacaaactgccaggaagcataaagcactacgcaaagagggcgttgaaatccattgtccctattgctcaagaatatggagtaaactacgcaaaggccaagaatcctcgtaatcaaatcaaactgactgtagctgttgctactgagacaagcatgaatattgtgctgaacacaccaaaggcaatcgtttacaagcgtggggtgtgtctacctgttgctttaccaattggaaacactgctgccgagctgcaagcgacccgggacaactgggctgacaagatgtcctatttggttaccaaagctaacgcagttgaatgctccctcatcaacaacacactgaccacattcaacaacaggaaagctagagatgagctgccacactcgtgctaccaggtcttggctcaggattgcacaccagaactcaaattcatggttctgctgaagaaagaccaaatacaggatcagaaccagatcaatgttaagatttcagacatcgatgtggacatgtatcggaagaacaacgccattgcggtgatggttaacggagttgaaatccctaacagcaacctgccatacctgcatccatcaggtaacatacatataagacagtcaaatgaaggcattactctcaatgcacccagccatggtcttcaggaggtcttccttggcttcaacgagctgagggttaaagttgcagactggatgaaaggaaagacttgtggtgcctgtggaacggcaagcggaaatgtcggagacgagtaccgcacacccagtgaacaggtgaccaaggatgccatcagctacgcccactcctgggttctgtcttcaaacacctgccgtgatccctccgagtgttccatcaagcaggaatctgtgaagctggagaagcgggtgatctttgaaggtgtggagtccaaatgctactctgttgagcccgtgctgcaatgcctgcccggctgtatcccagtgagaaccactaccgtcaacgttggctttcactgcctgcccagtgacacaactgtggaccgttctggtctgagcagcttctttgagaagagcatcgacctgagggatactgccgaagcccacctggcctgtcgctgcactcctcagtgtgctgagttcatggtttctaaaggtgaagaattgttcactggtgttgttccaattttggttgaattggatggtgatgttaatggtcataaattctctgtttctggtgaaggtgaaggtgatgctacttatggtaaattgactttgaaattcatttgtactactggtaaattgccagttccatggccaactttggttactactttgacttatggtgttcaatgtttctccagatatccagatcatatgaaacaacatgatttcttcaaatctgctatgccagaaggttatgttcaagaaagaactattttcttcaaagatgatggtaattataaaactagagctgaagttaaattcgaaggtgatactttggttaatagaattgaattgaaaggtattgatttcaaagaagatggtaatattttgggtcataaattggaatataattataattctcataatgtttatattatggctgataaacaaaaaaatggtattaaagttaatttcaaaattagacataatattgaagatggttctgttcaattggctgatcattatcaacaaaatactccaattggtgatggtccagttttgttgccagataatcattatttgtctactcaatctgctttgtctaaagatccaaatgaaaaaagagatcatatggttttgttggagttcgttactgctgctggtattactttgggtatggatgaattgtataaatctggtttgaggtccagagctcaagcttctaattctgctgttgatggtactgctggtccaggttctactggttccagagcttgtaaaaattggttctcttctttgtctcatttcgttattcatttgtaactactagtagcggccgctgcaggaattc
 
  
  

Revision as of 11:20, 22 October 2016


pGAP-Vitellogenin-GFP device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1905
    Illegal NheI site found at 2139
    Illegal NheI site found at 4457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 3274
    Illegal BglII site found at 3900
    Illegal BglII site found at 3936
    Illegal BamHI site found at 1281
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI site found at 3681
    Illegal BsaI site found at 3811
    Illegal SapI site found at 244
    Illegal SapI site found at 3868
    Illegal SapI.rc site found at 5732


Design Notes

Mutagenesis of UCL iGEM 2009 BBa_K239009.

The following is the sequence of Vitellogenin proposal sequence:


48% GC

Source

UCL iGEM 2009.

References