Difference between revisions of "Part:BBa K1983000:Design"

(→‎Primers)
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===Source===
 
===Source===
  
−
E coli
+
This part is an improvement of ([https://parts.igem.org/Part:BBa_K1692004 BBa_K1692004]) and was synthesized by Integrate DNA Technologies
  
 
===References===
 
===References===

Latest revision as of 10:37, 22 October 2016


Codon optimized AvPAL with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1265
    Illegal XhoI site found at 1702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:

PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG

Primers used for colony PCR screening:

For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC

For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG

Primers used for cloning into biobrick:

PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg
PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG

Source

This part is an improvement of (BBa_K1692004) and was synthesized by Integrate DNA Technologies

References