Difference between revisions of "Part:BBa K1943023:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
fdsf
+
This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.
 
+
 
+
  
 
===Source===
 
===Source===
  
fdsf
+
Original TRPC5:
 +
Sequence from NCBI
 +
Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.
  
 
===References===
 
===References===

Revision as of 03:24, 22 October 2016


TRPC5-12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2539
    Illegal BsaI.rc site found at 1627
    Illegal BsaI.rc site found at 2054
    Illegal SapI site found at 1722


Design Notes

This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.

Source

Original TRPC5: Sequence from NCBI Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.

References