Difference between revisions of "Part:BBa K1943008:Design"

(Design Notes)
Line 11: Line 11:
 
===Source===
 
===Source===
  
sfGFP
+
sfGFP coding sequence was our instructor, Huangwei's lab.
  
 
===References===
 
===References===

Revision as of 02:46, 22 October 2016


sfGFP, green fluorescence protein reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. We design primer and do PCR of this sfGFP coding device and ligate it to pSB1C3 backbone.

Source

sfGFP coding sequence was our instructor, Huangwei's lab.

References