Difference between revisions of "Part:BBa K1943001"
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<partinfo>BBa_K1943001 short</partinfo> | <partinfo>BBa_K1943001 short</partinfo> | ||
| − | We successfully constructed eight plasmids with various pigment coding sequence, including eforRed, gfasPurple, Fw Yellow and SpisPink. | + | We successfully constructed eight plasmids with various pigment coding sequence, including <partinfo>BBa_K592012</partinfo>eforRed, <partinfo>BBa_K1033919</partinfo>gfasPurple, <partinfo>BBa_K1033910</partinfo>Fw Yellow and <partinfo>BBa_K1033932</partinfo>SpisPink.<br> |
| − | Compared to the common used fluorescence protein such as GFP, RFP, chromoprotein is significantly more useful reporter gene. In our parts, the coding sequences of these pigments are all among 700bp long, and the expression efficiency is high according to the experiment results. | + | Compared to the common used fluorescence protein such as GFP, RFP, chromoprotein is significantly more useful reporter gene. In our parts, the coding sequences of these pigments are all among 700bp long, and the expression efficiency is high according to the experiment results.<br> |
| − | We contributed this series of the chromoprotein plasmid for the reason that they have significant advantages to be used as backbones. The length of 700bp is easy to distinguish from the original backbone (around 2000bp) by gel electrophoresis when constructing new expression system. Additionally, this kind of backbones are superior because we are able to directly observe the expression results by using naked eyes. It will be much more convenient for the laboratory with limited conditions and also save our time. In the tests, the color of colonies were easy to recognize after 18 hours of incubation. | + | We contributed this series of the chromoprotein plasmid for the reason that they have significant advantages to be used as backbones. The length of 700bp is easy to distinguish from the original backbone (around 2000bp) by gel electrophoresis when constructing new expression system. Additionally, this kind of backbones are superior because we are able to directly observe the expression results by using naked eyes. It will be much more convenient for the laboratory with limited conditions and also save our time. In the tests, the color of colonies were easy to recognize after 18 hours of incubation.<br> |
| − | Our plasmid can also be a substitute for | + | Our plasmid can also be a substitute for <partinfo>BBa_J04450</partinfo> , which kindly recommended to be used in plasmid construction by replacing the RFP gene. For the same reason, we provide these pigment sequence to make the construction process more efficiently. <br> |
| − | < | + | |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[File: | + | [[File:108&118.jpeg|center]] |
| − | Right is BBa_K1943000(Strong Promoter & RBS), Left is BBa_K1943001(Weak Promoter & RBS) | + | Right is <partinfo>BBa_K1943000</partinfo>(Strong Promoter & RBS), Left is <partinfo>BBa_K1943001</partinfo>(Weak Promoter & RBS) |
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[[File:SUSTech_Pattern_of_chromoprotein_1.jpeg]] | [[File:SUSTech_Pattern_of_chromoprotein_1.jpeg]] | ||
| − | A few of the chromoproteins form the pattern of S(BBa_K1943000), U(BBa_K1943002), S(BBa_K1943004), T(BBa_K1943006), e(BBa_K1943005), c(BBa_K1943003), h(BBa_K1943001), !(BBa_K1943007) | + | [[File:SUSTech_Shenzhen-SUSTecho.png|center]] |
| + | A few of the chromoproteins form the pattern of S(<partinfo>BBa_K1943000</partinfo>), U(<partinfo>BBa_K1943002</partinfo>), S(<partinfo>BBa_K1943004</partinfo>), T(<partinfo>BBa_K1943006</partinfo>), e(<partinfo>BBa_K1943005</partinfo>), c(<partinfo>BBa_K1943003</partinfo>), h(<partinfo>BBa_K1943001</partinfo>), !(<partinfo>BBa_K1943007</partinfo>)<br><br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 01:07, 22 October 2016
fwYellow, yellow chromoprotein reporter system (medium Promoter , weak RBS)
We successfully constructed eight plasmids with various pigment coding sequence, including BBa_K592012eforRed, BBa_K1033919gfasPurple, BBa_K1033910Fw Yellow and BBa_K1033932SpisPink.
Compared to the common used fluorescence protein such as GFP, RFP, chromoprotein is significantly more useful reporter gene. In our parts, the coding sequences of these pigments are all among 700bp long, and the expression efficiency is high according to the experiment results.
We contributed this series of the chromoprotein plasmid for the reason that they have significant advantages to be used as backbones. The length of 700bp is easy to distinguish from the original backbone (around 2000bp) by gel electrophoresis when constructing new expression system. Additionally, this kind of backbones are superior because we are able to directly observe the expression results by using naked eyes. It will be much more convenient for the laboratory with limited conditions and also save our time. In the tests, the color of colonies were easy to recognize after 18 hours of incubation.
Our plasmid can also be a substitute for BBa_J04450 , which kindly recommended to be used in plasmid construction by replacing the RFP gene. For the same reason, we provide these pigment sequence to make the construction process more efficiently.
Usage and Biology
Right is BBa_K1943000(Strong Promoter & RBS), Left is BBa_K1943001(Weak Promoter & RBS)
A few of the chromoproteins form the pattern of S(BBa_K1943000), U(BBa_K1943002), S(BBa_K1943004), T(BBa_K1943006), e(BBa_K1943005), c(BBa_K1943003), h(BBa_K1943001), !(BBa_K1943007)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 172
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 549