Difference between revisions of "Part:BBa K530001"
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| − | This part has been improved. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2117012"> | + | |
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| + | This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site AgeI at bp 202, making the part compatible with the RFC25 standard. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2117012">BBa_K2117012</a> </html> for details of the new and improved part. | ||
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Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene. | Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene. | ||
Latest revision as of 21:30, 21 October 2016
CRTE
This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site AgeI at bp 202, making the part compatible with the RFC25 standard. Please see BBa_K2117012 for details of the new and improved part.
Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 335
Illegal XhoI site found at 34
Illegal XhoI site found at 166
Illegal XhoI site found at 985 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 202
- 1000COMPATIBLE WITH RFC[1000]
Characterization of performance
Below we can see the beta-carotene production levels on a per cell basis for different combinations of parts involved in beta-carotene synthesis. These are compared with a wild type control. tHMG1 is used to funnel more initial substrate into the pathway and although useful, was not submitted as a part. 2I indicates that two copies of crtI were used.
Plasmid Map
Sequencing
This is the sequencing for colony 4.