Difference between revisions of "Part:BBa K530002"
| (One intermediate revision by the same user not shown) | |||
| Line 3: | Line 3: | ||
<html> | <html> | ||
| − | This part has been improved. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search= | + | This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site BgIII at bp 204, making the part compatible with the RFC21 standard. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2117013">BBa_K2117013</a> </html> for details of the new and improved part. |
| + | |||
| + | |||
| + | |||
Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene. | Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene. | ||
Latest revision as of 21:28, 21 October 2016
CRTI
This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site BgIII at bp 204, making the part compatible with the RFC21 standard. Please see BBa_K2117013 for details of the new and improved part.
Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 204
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1108
Illegal SapI site found at 508
Characterization of performance
Below we can see the beta-carotene production levels on a per cell basis for different combinations of parts involved in beta-carotene synthesis. These are compared with a wild type control. tHMG1 is used to funnel more initial substrate into the pathway and although useful, was not submitted as a part. 2I indicates that two copies of crtI were used.
Plasmid Map
Sequencing
This is the sequencing for colony 7.