Difference between revisions of "Part:BBa K1954002:Design"
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Mutagenesis of UCL iGEM 2009 BBa_K239009. | Mutagenesis of UCL iGEM 2009 BBa_K239009. | ||
| + | The following is the sequence of Vitellogenin proposal sequence: | ||
| + | gaattcgcggccgcttctagagagatcttttttgtagaaatgtcttggtgtcctcgtccaatcaggtagccatctctgaaatatctggctccgttgcaactccgaacgacctgctggcaacgtaaaattctccggggtaaaacttaaatgtggagtaatggaaccagaaacgtctcttcccttctctctccttccaccgcccgttaccgtccctaggaaattttactctgctggagagcttcttctacggcccccttgcagcaatgctcttcccagcattacgttgcgggtaaaacggaggtcgtgtacccgacctagcagcccagggatggaaaagtcccggccgtcgctggcaataatagcgggcggacgcatgtcatgagattattggaaaccaccagaatcgaatataaaaggcgaacacctttcccaattttggtttctcctgacccaaagactttaaatttaatttatttgtccctatttcaatcaattgaacaactaatacaaatgttggccccaggattcgcctctgttaagacctacatgtacaaatatgaagcggttcttatgggcggcctgcctgaagagggcctggctcgagctggggttaaaatccggggcaaagttttgatcagtgcaacaagtgccaacgactacattctgaagcttgtagaccctcagttgctggagtacagtggcatctggcccaaagatcctttccatccagccaccaagctcaccacagccctggctactcagctctcgacaccgatcaagtttgagtatacaaacggcgttgtcgggagactggctgcacctcctggggtctccacaacagtgctgaatatctacaggggaatcatcaacctcctgcaactgaatgtaaagaagacacagaacgtctacgagatgcaagagtctggagctcatggtgtgtgcaagaccaactatgtgatcagggaggacgcgagagccgaacgcattcatctgaccaagaccaaggacctgaaccactgccaggagaaaatcatgaaggccatcggcttggaacacgtagagaaatgccatgattgtgaagctagaggaaagagcctgaagggaactgcttcctataactacatcatgaagccagcacccagtggttctctgattatggaggctgtcgctagagaggtcatcgagttctcacctttcaacattttgaatggcgctgctcagatggagtctaagcaaattctgaccttcctggatatcgagaacacccctgtggatcatgccagatacacctatgttcaccgcggatccctgcaatatgagcatggcagcgagattctccagacacccatccatcttctgagggtcacccatgccgaggctcagattgtcagcactctgaaccacctggtagcctccaacgtggccaaggtccatgaagatgcccctctgaagtttgttgagctcatccaggtgatgcgtgtggccagatttgagactattgagtccctctgggctcagtttaaatccagacctgatcacaggtactggttactgaatgctgtcccccacattcgcactcacgctgcgcttaagttcctcattgagaagctccttgctaatgagttaagtgagactgaagctgctatggctctcttggaatgtctgcactctgtgacagctgaccagaaaaccattgaacttgtcagaagcctggctgagaaccacagagtgaaacgtaacgctgtgctcaacgagattgtgatgctgggctggggcactgtaatttccaggttctgtaaagcgcagccatcttgctcatctgatcttgtgacacctgtacatagacaagttgcagaggctgttgaaactggtgacatcgatcagctcactgtcacgctcaaatgcctggataacgctggacatcctgctagcattaagacaatcatgaagttcctgcctggctttggcagtgctgctgcccgagtcccactcaaagttcaggttgacgctgttctagccctgaggagaattgcaaagagggaacccaagatggtccaggaaatagctgctcagttgctcatggaaaagcatctccatgcagaactgcgtatggttgctgccatggtgctctttgagactaaactccccgtgggtctagcagctagcatttccacagccttgatcaaagaaaagaacctgcaagtcgttagctttgtctactcttacatgaaggccatggccaagaccacatcccctgaccacgtttctgttgctgccgcatgtaatgttgccttgaggttcctcaaccccaaattaggcagactgaacttccgctacagccgagccttccatgtggatacctataacaatgcctggatgatgggtgctgccgccagtgccgtcttaattaacgacgctgcaaccgtgttaccaagaatgattatggccaaagcccgtacttacatggccggagcttatgttgatgcttttgaggttggagtgaggactgagggaatccaggaggctcttttgaaaagacgacatgaaaattctgagaatgcagacaggatcaccaagattaaacaagccatgagagctctttctgagtggagggctaatccttcgagccaggccctggcctctatgtatgtgaaggtcttcggacaagaaattgcatttgccaacattgacaaatccaaggttgaccagcttatccagtttgccagtggacctttgagaaacgtattcagagatgctgtgaactctgtgctgtctggttatgcaacacattttgctaaaccaatgctgctcggtgagctccgtctcatccttcccaccactgttgggttgcccatggagatcagcctcattacatccgctgtgactgctgcatctgttgacgtccaagccactgtgtcaccacctctgcctgtcaactaccgagtttcccagcttctggagtccgatatccaactgagggctacagttgctccaagtcttgccatgcagacctatgcattcatgggtgtgaacaccgccttaatccaggctgccgtgatgacaaaagccaaagtttacacagctgttcctgcacagataaaagcaaggattgacattgttaagggcaacttgaaggttgagttcctgtctctccagggcattaacacaattgcatctgcacatgcggagacggttgccattgcaagaaatgtggaagacctcccagccgcaagaagcacaccactgatctcatctgaaactgcatcacaactttcaaaggcctctctcaactcaaagatctccaggatggcatcctctgtgactggtggcatgtctgcgtcatctgaaatcattcctgctgacctgccaagtaagattgggaggaaaatgaaactccctaaaacctacaggaagaaaatccgtgcttcaagcagaatgctaggattcaaggcctacgctgagattgaatctcacaatgccgcctacatcagagactgccctctctacgctctgatcggaaagcatgctgcttctgttaggattgctccagcttctggaccagtcattgagaagattgaagttgagattcaggtcggagataaagcagcagaaaatatgattaaagcgattgacatgagcgaagaggaggaagctcttgaggataagaatgtcctcttgaaaatcaagaaaatactggcacctggtctcaagaacaccacatcatcttcctccagctcctccagctcctcttcatccagctctagctccaacaagtcttcttcatccagttcccgctccagcagctcccagtcatccagctctcgttcccataggtctcgctccagaaagtcccagtctagcagctctcagtcaagccgctctccctcaagctcttcctcctcttcctcctcttcatcatccagatcttcttccaggtcatcttccagatcatcttccagatcttcttctaggtcctcctctcgctccagaactaagatggctgacattgttgctcctattatcacgacgtccaccagagtgagcagttcctccagtcgatcagcctctaacagctcctccagcagtgcttcatacttgctcagctcatctaaaagaagaagcagaagcagaagcagcagcagcagtagcagcagcagcagcagcagcagcagtagcagcagcagcagcagcagcagtagcaagaacagcaagcgcagcaagagcagcaacagcaagtcatcaagctctaggtcctctcggcgcagtgctcagtctaagcaacaactgcttgccttgaagttcagaaagaaccacgtccacaggcatgccatctccacacagcgcggcagcagtcacagcagtgcccgcagcttcgattccatctacaataaggccaagtacctcgctaacacactcactcctgccatgtccattgcaatccgtgccgtgagagtcgaccacaaggtccagggataccagctagcagcttacctggacaaacagaccaatagactgcaattgatttttgccagagtcgctgagaaggacaactggagaatctgtgccgacattgtgcagctgagttcgcacaagctgatggccaagactgcctggggtgctgaatgcaagcaatactccaccatgattgtagctgaaactggtcttttgggtcatgagcccgcagcccgcttgaagctgacctgggacaaactgccaggaagcataaagcactacgcaaagagggcgttgaaatccattgtccctattgctcaagaatatggagtaaactacgcaaaggccaagaatcctcgtaatcaaatcaaactgactgtagctgttgctactgagacaagcatgaatattgtgctgaacacaccaaaggcaatcgtttacaagcgtggggtgtgtctacctgttgctttaccaattggaaacactgctgccgagctgcaagcgacccgggacaactgggctgacaagatgtcctatttggttaccaaagctaacgcagttgaatgctccctcatcaacaacacactgaccacattcaacaacaggaaagctagagatgagctgccacactcgtgctaccaggtcttggctcaggattgcacaccagaactcaaattcatggttctgctgaagaaagaccaaatacaggatcagaaccagatcaatgttaagatttcagacatcgatgtggacatgtatcggaagaacaacgccattgcggtgatggttaacggagttgaaatccctaacagcaacctgccatacctgcatccatcaggtaacatacatataagacagtcaaatgaaggcattactctcaatgcacccagccatggtcttcaggaggtcttccttggcttcaacgagctgagggttaaagttgcagactggatgaaaggaaagacttgtggtgcctgtggaacggcaagcggaaatgtcggagacgagtaccgcacacccagtgaacaggtgaccaaggatgccatcagctacgcccactcctgggttctgtcttcaaacacctgccgtgatccctccgagtgttccatcaagcaggaatctgtgaagctggagaagcgggtgatctttgaaggtgtggagtccaaatgctactctgttgagcccgtgctgcaatgcctgcccggctgtatcccagtgagaaccactaccgtcaacgttggctttcactgcctgcccagtgacacaactgtggaccgttctggtctgagcagcttctttgagaagagcatcgacctgagggatactgccgaagcccacctggcctgtcgctgcactcctcagtgtgctgagttcatggtttctaaaggtgaagaattgttcactggtgttgttccaattttggttgaattggatggtgatgttaatggtcataaattctctgtttctggtgaaggtgaaggtgatgctacttatggtaaattgactttgaaattcatttgtactactggtaaattgccagttccatggccaactttggttactactttgacttatggtgttcaatgtttctccagatatccagatcatatgaaacaacatgatttcttcaaatctgctatgccagaaggttatgttcaagaaagaactattttcttcaaagatgatggtaattataaaactagagctgaagttaaattcgaaggtgatactttggttaatagaattgaattgaaaggtattgatttcaaagaagatggtaatattttgggtcataaattggaatataattataattctcataatgtttatattatggctgataaacaaaaaaatggtattaaagttaatttcaaaattagacataatattgaagatggttctgttcaattggctgatcattatcaacaaaatactccaattggtgatggtccagttttgttgccagataatcattatttgtctactcaatctgctttgtctaaagatccaaatgaaaaaagagatcatatggttttgttggagttcgttactgctgctggtattactttgggtatggatgaattgtataaatctggtttgaggtccagagctcaagcttctaattctgctgttgatggtactgctggtccaggttctactggttccagagcttgtaaaaattggttctcttctttgtctcatttcgttattcatttgtaactactagtagcggccgctgcaggaattc | ||
===Source=== | ===Source=== | ||
Revision as of 17:35, 21 October 2016
pGAP-Vitellogenin-GFP device
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1905
Illegal NheI site found at 2139
Illegal NheI site found at 4457 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 3274
Illegal BglII site found at 3900
Illegal BglII site found at 3936
Illegal BamHI site found at 1281
Illegal XhoI site found at 577 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 806
Illegal BsaI site found at 3681
Illegal BsaI site found at 3811
Illegal SapI site found at 244
Illegal SapI site found at 3868
Illegal SapI.rc site found at 5732
Design Notes
Mutagenesis of UCL iGEM 2009 BBa_K239009.
The following is the sequence of Vitellogenin proposal sequence:
gaattcgcggccgcttctagagagatcttttttgtagaaatgtcttggtgtcctcgtccaatcaggtagccatctctgaaatatctggctccgttgcaactccgaacgacctgctggcaacgtaaaattctccggggtaaaacttaaatgtggagtaatggaaccagaaacgtctcttcccttctctctccttccaccgcccgttaccgtccctaggaaattttactctgctggagagcttcttctacggcccccttgcagcaatgctcttcccagcattacgttgcgggtaaaacggaggtcgtgtacccgacctagcagcccagggatggaaaagtcccggccgtcgctggcaataatagcgggcggacgcatgtcatgagattattggaaaccaccagaatcgaatataaaaggcgaacacctttcccaattttggtttctcctgacccaaagactttaaatttaatttatttgtccctatttcaatcaattgaacaactaatacaaatgttggccccaggattcgcctctgttaagacctacatgtacaaatatgaagcggttcttatgggcggcctgcctgaagagggcctggctcgagctggggttaaaatccggggcaaagttttgatcagtgcaacaagtgccaacgactacattctgaagcttgtagaccctcagttgctggagtacagtggcatctggcccaaagatcctttccatccagccaccaagctcaccacagccctggctactcagctctcgacaccgatcaagtttgagtatacaaacggcgttgtcgggagactggctgcacctcctggggtctccacaacagtgctgaatatctacaggggaatcatcaacctcctgcaactgaatgtaaagaagacacagaacgtctacgagatgcaagagtctggagctcatggtgtgtgcaagaccaactatgtgatcagggaggacgcgagagccgaacgcattcatctgaccaagaccaaggacctgaaccactgccaggagaaaatcatgaaggccatcggcttggaacacgtagagaaatgccatgattgtgaagctagaggaaagagcctgaagggaactgcttcctataactacatcatgaagccagcacccagtggttctctgattatggaggctgtcgctagagaggtcatcgagttctcacctttcaacattttgaatggcgctgctcagatggagtctaagcaaattctgaccttcctggatatcgagaacacccctgtggatcatgccagatacacctatgttcaccgcggatccctgcaatatgagcatggcagcgagattctccagacacccatccatcttctgagggtcacccatgccgaggctcagattgtcagcactctgaaccacctggtagcctccaacgtggccaaggtccatgaagatgcccctctgaagtttgttgagctcatccaggtgatgcgtgtggccagatttgagactattgagtccctctgggctcagtttaaatccagacctgatcacaggtactggttactgaatgctgtcccccacattcgcactcacgctgcgcttaagttcctcattgagaagctccttgctaatgagttaagtgagactgaagctgctatggctctcttggaatgtctgcactctgtgacagctgaccagaaaaccattgaacttgtcagaagcctggctgagaaccacagagtgaaacgtaacgctgtgctcaacgagattgtgatgctgggctggggcactgtaatttccaggttctgtaaagcgcagccatcttgctcatctgatcttgtgacacctgtacatagacaagttgcagaggctgttgaaactggtgacatcgatcagctcactgtcacgctcaaatgcctggataacgctggacatcctgctagcattaagacaatcatgaagttcctgcctggctttggcagtgctgctgcccgagtcccactcaaagttcaggttgacgctgttctagccctgaggagaattgcaaagagggaacccaagatggtccaggaaatagctgctcagttgctcatggaaaagcatctccatgcagaactgcgtatggttgctgccatggtgctctttgagactaaactccccgtgggtctagcagctagcatttccacagccttgatcaaagaaaagaacctgcaagtcgttagctttgtctactcttacatgaaggccatggccaagaccacatcccctgaccacgtttctgttgctgccgcatgtaatgttgccttgaggttcctcaaccccaaattaggcagactgaacttccgctacagccgagccttccatgtggatacctataacaatgcctggatgatgggtgctgccgccagtgccgtcttaattaacgacgctgcaaccgtgttaccaagaatgattatggccaaagcccgtacttacatggccggagcttatgttgatgcttttgaggttggagtgaggactgagggaatccaggaggctcttttgaaaagacgacatgaaaattctgagaatgcagacaggatcaccaagattaaacaagccatgagagctctttctgagtggagggctaatccttcgagccaggccctggcctctatgtatgtgaaggtcttcggacaagaaattgcatttgccaacattgacaaatccaaggttgaccagcttatccagtttgccagtggacctttgagaaacgtattcagagatgctgtgaactctgtgctgtctggttatgcaacacattttgctaaaccaatgctgctcggtgagctccgtctcatccttcccaccactgttgggttgcccatggagatcagcctcattacatccgctgtgactgctgcatctgttgacgtccaagccactgtgtcaccacctctgcctgtcaactaccgagtttcccagcttctggagtccgatatccaactgagggctacagttgctccaagtcttgccatgcagacctatgcattcatgggtgtgaacaccgccttaatccaggctgccgtgatgacaaaagccaaagtttacacagctgttcctgcacagataaaagcaaggattgacattgttaagggcaacttgaaggttgagttcctgtctctccagggcattaacacaattgcatctgcacatgcggagacggttgccattgcaagaaatgtggaagacctcccagccgcaagaagcacaccactgatctcatctgaaactgcatcacaactttcaaaggcctctctcaactcaaagatctccaggatggcatcctctgtgactggtggcatgtctgcgtcatctgaaatcattcctgctgacctgccaagtaagattgggaggaaaatgaaactccctaaaacctacaggaagaaaatccgtgcttcaagcagaatgctaggattcaaggcctacgctgagattgaatctcacaatgccgcctacatcagagactgccctctctacgctctgatcggaaagcatgctgcttctgttaggattgctccagcttctggaccagtcattgagaagattgaagttgagattcaggtcggagataaagcagcagaaaatatgattaaagcgattgacatgagcgaagaggaggaagctcttgaggataagaatgtcctcttgaaaatcaagaaaatactggcacctggtctcaagaacaccacatcatcttcctccagctcctccagctcctcttcatccagctctagctccaacaagtcttcttcatccagttcccgctccagcagctcccagtcatccagctctcgttcccataggtctcgctccagaaagtcccagtctagcagctctcagtcaagccgctctccctcaagctcttcctcctcttcctcctcttcatcatccagatcttcttccaggtcatcttccagatcatcttccagatcttcttctaggtcctcctctcgctccagaactaagatggctgacattgttgctcctattatcacgacgtccaccagagtgagcagttcctccagtcgatcagcctctaacagctcctccagcagtgcttcatacttgctcagctcatctaaaagaagaagcagaagcagaagcagcagcagcagtagcagcagcagcagcagcagcagcagtagcagcagcagcagcagcagcagtagcaagaacagcaagcgcagcaagagcagcaacagcaagtcatcaagctctaggtcctctcggcgcagtgctcagtctaagcaacaactgcttgccttgaagttcagaaagaaccacgtccacaggcatgccatctccacacagcgcggcagcagtcacagcagtgcccgcagcttcgattccatctacaataaggccaagtacctcgctaacacactcactcctgccatgtccattgcaatccgtgccgtgagagtcgaccacaaggtccagggataccagctagcagcttacctggacaaacagaccaatagactgcaattgatttttgccagagtcgctgagaaggacaactggagaatctgtgccgacattgtgcagctgagttcgcacaagctgatggccaagactgcctggggtgctgaatgcaagcaatactccaccatgattgtagctgaaactggtcttttgggtcatgagcccgcagcccgcttgaagctgacctgggacaaactgccaggaagcataaagcactacgcaaagagggcgttgaaatccattgtccctattgctcaagaatatggagtaaactacgcaaaggccaagaatcctcgtaatcaaatcaaactgactgtagctgttgctactgagacaagcatgaatattgtgctgaacacaccaaaggcaatcgtttacaagcgtggggtgtgtctacctgttgctttaccaattggaaacactgctgccgagctgcaagcgacccgggacaactgggctgacaagatgtcctatttggttaccaaagctaacgcagttgaatgctccctcatcaacaacacactgaccacattcaacaacaggaaagctagagatgagctgccacactcgtgctaccaggtcttggctcaggattgcacaccagaactcaaattcatggttctgctgaagaaagaccaaatacaggatcagaaccagatcaatgttaagatttcagacatcgatgtggacatgtatcggaagaacaacgccattgcggtgatggttaacggagttgaaatccctaacagcaacctgccatacctgcatccatcaggtaacatacatataagacagtcaaatgaaggcattactctcaatgcacccagccatggtcttcaggaggtcttccttggcttcaacgagctgagggttaaagttgcagactggatgaaaggaaagacttgtggtgcctgtggaacggcaagcggaaatgtcggagacgagtaccgcacacccagtgaacaggtgaccaaggatgccatcagctacgcccactcctgggttctgtcttcaaacacctgccgtgatccctccgagtgttccatcaagcaggaatctgtgaagctggagaagcgggtgatctttgaaggtgtggagtccaaatgctactctgttgagcccgtgctgcaatgcctgcccggctgtatcccagtgagaaccactaccgtcaacgttggctttcactgcctgcccagtgacacaactgtggaccgttctggtctgagcagcttctttgagaagagcatcgacctgagggatactgccgaagcccacctggcctgtcgctgcactcctcagtgtgctgagttcatggtttctaaaggtgaagaattgttcactggtgttgttccaattttggttgaattggatggtgatgttaatggtcataaattctctgtttctggtgaaggtgaaggtgatgctacttatggtaaattgactttgaaattcatttgtactactggtaaattgccagttccatggccaactttggttactactttgacttatggtgttcaatgtttctccagatatccagatcatatgaaacaacatgatttcttcaaatctgctatgccagaaggttatgttcaagaaagaactattttcttcaaagatgatggtaattataaaactagagctgaagttaaattcgaaggtgatactttggttaatagaattgaattgaaaggtattgatttcaaagaagatggtaatattttgggtcataaattggaatataattataattctcataatgtttatattatggctgataaacaaaaaaatggtattaaagttaatttcaaaattagacataatattgaagatggttctgttcaattggctgatcattatcaacaaaatactccaattggtgatggtccagttttgttgccagataatcattatttgtctactcaatctgctttgtctaaagatccaaatgaaaaaagagatcatatggttttgttggagttcgttactgctgctggtattactttgggtatggatgaattgtataaatctggtttgaggtccagagctcaagcttctaattctgctgttgatggtactgctggtccaggttctactggttccagagcttgtaaaaattggttctcttctttgtctcatttcgttattcatttgtaactactagtagcggccgctgcaggaattc
Source
UCL iGEM 2009.