Difference between revisions of "Part:BBa K2088672"
 
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<partinfo>BBa_K2088672 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2088672 SequenceAndFeatures</partinfo>
  
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[[Image:http://NAU_CNINA_composite_part_Fig1.png|tuhumb|500px|center|Figure1|3-HBA was degraded thoroughly by our engineered E.coli transformed with this composite part.]]
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[[Image:NAU_CNINA_composite_Fig1.png|thumb|500px|center|Figure1:3-HBA was degraded thoroughly by our engineered E.coli transformed with this composite part.]]
  
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[[Image:http://NAU_CNINA_composite_part_Fig2.png|tuhumb|500px|center|Figure2|The ordinate is the concentration of 3-HBA. The left column shows the figure of Klebsiella pneumoniae M5a1, while the right one shows the figure of our engineered bacteria. From this figure we can find that our engineered bacteria can degrade 3-HBA much faster than M5a1 in 24h.]]
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[[Image:NAU_CNINA_composite_Fig2.png|thumb|500px|center|Figure2:The ordinate is the concentration of 3-HBA. The left column shows the figure of Klebsiella pneumoniae M5a1, while the right one shows the figure of our engineered bacteria. From this figure we can find that our engineered bacteria can degrade 3-HBA much faster than M5a1 in 24h.]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 13:55, 21 October 2016


copA + riboJ +mhbDHIM + terminator

The composite part contains copA promoter, riboJ , a gene cluster mhbDHIM , and a terminator. The promoter is induced by copper ion and transcribe downstream genes. The riboJ between copA and the gene cluster can decrease interference of one part type （promoter）on another（protein coding sequence）. The gene cluster mhbDHIM contains four connected genes which can degrade 3-hydroxybenzoate to the Fumarate and Pyruvate step by step.

After experienting, we found that the device can degrade the 3-hydroxybenzoate completely in short time. Also, engineering bacteria with the device grows better in the medium with 3-hydroxybenzoate than Klebsiella pneumoniae M5a1which is capable of utilizing 3-hydroxybenzoate via gentisate, and can tolerate higher concentration of 3- hydroxybenzoate.In conclusion, the device can help the E.coli degrade the 3- hydroxybenzoate effectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2518
    Illegal BamHI site found at 2904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 2708
    Illegal NgoMIV site found at 3845
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3038
Figure1:3-HBA was degraded thoroughly by our engineered E.coli transformed with this composite part.
Figure2:The ordinate is the concentration of 3-HBA. The left column shows the figure of Klebsiella pneumoniae M5a1, while the right one shows the figure of our engineered bacteria. From this figure we can find that our engineered bacteria can degrade 3-HBA much faster than M5a1 in 24h.