Difference between revisions of "Part:BBa K1992010"
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| − | For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>) | + | For methods and results please go to PctA-Tar (<partinfo>BBa_K1992001</partinfo>) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page |
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Latest revision as of 13:52, 21 October 2016
PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 331
Illegal NgoMIV site found at 412
Illegal NgoMIV site found at 416
Illegal NgoMIV site found at 435
Illegal AgeI site found at 535
Illegal AgeI site found at 746
Illegal AgeI site found at 1601 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2657
Introduction
A GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.
Usage and Biology
This expression device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane.
Experiments and results
For methods and results please go to PctA-Tar (BBa_K1992001) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page