Difference between revisions of "Part:BBa K2100029:Design"

 
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===References===
 
===References===
 +
Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.

Latest revision as of 13:41, 21 October 2016


pEXPR pERE6:eYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
    Illegal PstI site found at 581
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal PstI site found at 581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
    Illegal PstI site found at 581
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 18
    Illegal EcoRI site found at 333
    Illegal EcoRI site found at 343
    Illegal XbaI site found at 42
    Illegal PstI site found at 581
    Illegal AgeI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is from a synthetic promoter and a gene from a jellyfish's genome.

References

Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.