Difference between revisions of "Part:BBa K1992010"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | This device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane. | + | This expression device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane. |
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==Design considerations== | ==Design considerations== | ||
A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained. | A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained. | ||
Revision as of 10:32, 21 October 2016
PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 331
Illegal NgoMIV site found at 412
Illegal NgoMIV site found at 416
Illegal NgoMIV site found at 435
Illegal AgeI site found at 535
Illegal AgeI site found at 746
Illegal AgeI site found at 1601 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2657
Introduction
A GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.
Usage and Biology
This expression device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane.
Design considerations
A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained.
For methods and results please go to PctA-Tar (BBa_K1992001)