Difference between revisions of "Part:BBa K1974030"
 
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[[File:2016_NCTU-FORMOSA_GS.png|800px|thumb|center|'''Figure 1.'''GS Linker]]
 
[[File:2016_NCTU-FORMOSA_GS.png|800px|thumb|center|'''Figure 1.'''GS Linker]]
  
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This year, we have designed fusion proteins of spider toxin in conjunction with snowdrop lectin by the three-alanine linker. Through the reference searching, we found that the previous research all used the three-alanine linker. In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. So we went to the linker library in iGEM part registry website to search for a longer linker. At first, we planned to use the Flexible Peptide GS Linker(BBa_K416001) from 2010 NYU iGEM team. However, we found no result in the previous history using this linker. Therefore, we modified the sequence and added three more amino acids into the linker in case it was still too short for proper protein folding. In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.
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<h1>'''Introduction'''</h1>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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The GS Linker(BBa_K1974030) that we provided is a 18 amino acid. It is useful for expressing functional fusion proteins. So, we wanted to use it to concent the spider toxin peptide and snowdrop lectin.
 +
<h1>'''Modifying and Improving the Existed Biobrick'''</h1>
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<h3><b>Previous linker :
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K416001">BBa_K416001</a></html>
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of 2010 iGEM10_NYU</b></h3>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<br>
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GS Linker(BBa_K416001) is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. So we want to use it to concent the spider toxin peptide and snowdrop lectin.
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<br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Futrhermore, we found no result in the previous history using the Flexible Peptide GS Linker(<html><a href="https://parts.igem.org/Part:BBa_K416001">BBa_K416001</a></html>) from 2010 NYU iGEM team. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments. 
 +
 
 +
 
 +
<h1>'''Result and Experiment'''</h1>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.
 +
[[File:GS_linker_PCR_resullts.png|200px|thumb|center|'''Figure 2.'''GS linker.
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The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.]]
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<h1>'''More application of this part'''</h1>
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974033">BBa_K1974033</a></html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:18, 21 October 2016


GS linker

Figure 1.GS Linker

Introduction

       The GS Linker(BBa_K1974030) that we provided is a 18 amino acid. It is useful for expressing functional fusion proteins. So, we wanted to use it to concent the spider toxin peptide and snowdrop lectin.

Modifying and Improving the Existed Biobrick

Previous linker : BBa_K416001 of 2010 iGEM10_NYU

      
GS Linker(BBa_K416001) is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. So we want to use it to concent the spider toxin peptide and snowdrop lectin.
       In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Futrhermore, we found no result in the previous history using the Flexible Peptide GS Linker(BBa_K416001) from 2010 NYU iGEM team. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.


Result and Experiment

       In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.

Figure 2.GS linker. The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.

More application of this part

BBa_K1974033


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]