Difference between revisions of "Part:BBa K1555000:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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This sensor can be activated in low copper concentration and it's truely useful.
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how you used this part and how it worked out.
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===Applications of BBa_K1555000===
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[[File:2h 0mg.jpg]] 2h 0mg/L copper ion  [[File:2h 1mg.jpg]] 2h 1mg/L copper ion
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[[File:Copa.png]]
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[[File:Mcopa.png]]
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===Applications of BBa_K1555000-----NAU CHINA2016===
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<p>This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter( BBa_K2088015 copA+riboJ+B0030+GFP+B0015).For more details please check BBa_K2088015.</p>
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===User Reviews===
 
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==Experience of NAU-CHINA-2016 iGEM team--Kejian Shi ==
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====Experimental Design====
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To evaluate each site of the Anderson Promoter`s effect on the whole promoter, the NAU-CHINA Team used TASSEL5 to analyse the correlation degree between each mutation and the strength of the promoter.We combine the sequence and the data of strength , the P-Value is calculated using the GLM whose result is showed as the Manhadon.
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{|width='80%' style='border:1px solid gray'
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|-
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<partinfo>BBa_K1555000 AddReview 5</partinfo>
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<I>HSiTAIWAN</I>
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        <h2><strong>Detecting Copper in Chinese medicine</strong></h2>
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<h4>Objective</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.</p>
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<h4>Methods</h4>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fluorescence was measured at: <br>
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<ul>
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<li><em>Excitation peak: 470nm</em></li>
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<li><em>Emission peak: 508nm</em></li>
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</ul>
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every 30 minutes.
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<h4>Results</h4>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part is still specific to copper in Chinese medicine, so we can apply it in Chinese medicine examination.
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                <center>
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https://static.igem.org/mediawiki/parts/6/66/2016_hsitaiwan_cu_1.png
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                </center>
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<h4>Discussion</h4>
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        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper.
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<h4>Review</h4>
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This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.
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<!-- DON'T DELETE --><partinfo>BBa_K1555000 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1555000 EndReviews</partinfo>

Latest revision as of 07:05, 21 October 2016

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg.jpg 2h 0mg/L copper ion 2h 1mg.jpg 2h 1mg/L copper ion

Copa.png

Mcopa.png

Applications of BBa_K1555000-----NAU CHINA2016

This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter( BBa_K2088015 copA+riboJ+B0030+GFP+B0015).For more details please check BBa_K2088015.


User Reviews

UNIQ25d9e6356965653f-partinfo-00000000-QINU

•••••

HSiTAIWAN

Detecting Copper in Chinese medicine

Objective

     Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.

Methods

     Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).
     Fluorescence was measured at:

  • Excitation peak: 470nm
  • Emission peak: 508nm

every 30 minutes.

Results

     This part is still specific to copper in Chinese medicine, so we can apply it in Chinese medicine examination.

2016_hsitaiwan_cu_1.png

Discussion

     Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper.

Review

This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.


UNIQ25d9e6356965653f-partinfo-00000002-QINU