Difference between revisions of "Part:BBa K1555000:Experience"
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<p>This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter( BBa_K2088015 copA+riboJ+B0030+GFP+B0015).For more details please check BBa_K2088015.</p> | <p>This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter( BBa_K2088015 copA+riboJ+B0030+GFP+B0015).For more details please check BBa_K2088015.</p> | ||
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===User Reviews=== | ===User Reviews=== | ||
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| + | ==Experience of NAU-CHINA-2016 iGEM team--Kejian Shi == | ||
| + | ====Experimental Design==== | ||
| + | To evaluate each site of the Anderson Promoter`s effect on the whole promoter, the NAU-CHINA Team used TASSEL5 to analyse the correlation degree between each mutation and the strength of the promoter.We combine the sequence and the data of strength , the P-Value is calculated using the GLM whose result is showed as the Manhadon. | ||
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Revision as of 06:25, 21 October 2016
This sensor can be activated in low copper concentration and it's truely useful.
2h 0mg/L copper ion 
2h 1mg/L copper ion
Applications of BBa_K1555000
This year we add the RiboJ(a section of functional RNA used to buffer synthetic circuits from genetic context) between the copA pomoter and the RBS(B0030) as an improvement.Also we used GFP as the reporter( BBa_K2088015 copA+riboJ+B0030+GFP+B0015).For more details please check BBa_K2088015.
User Reviews
UNIQ71981e3de1002080-partinfo-00000000-QINU
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HSiTAIWAN |
Detecting Copper in Chinese medicineObjectiveMeasure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine. Methods Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).
every 30 minutes. ResultsThis part is still specific to copper in Chinese medicine, so we can apply it in Chinese medicine examination.
DiscussionSince E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper. ReviewThis part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter. |
