Difference between revisions of "Part:BBa K1955006"

 
 
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<b style="font-size:23px;">pSB1C3-5'HYG-OVA-3'UTR </b><br><br>
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OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.
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__NOTOC__
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<br><br>
<partinfo>BBa_K1955006 short</partinfo>
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<img src="https://static.igem.org/mediawiki/2016/2/2d/CGU_Taiwan--bio6.jpg" width=550px height=600px style="border:2px black solid;border-radius:8px;"></img><br><br>
 
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<img src="https://static.igem.org/mediawiki/2016/4/42/J4.jpeg" width=700px height=550px style="border:2px black solid;border-radius:8px;"></img><br><br>
OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:42, 21 October 2016

pSB1C3-5'HYG-OVA-3'UTR

OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.







Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1458
    Illegal BamHI site found at 3562
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2689