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| | ===Applications of BBa_K1996002=== | | ===Applications of BBa_K1996002=== |
| − | EtnR1 protein from <i>Mycobacterium</i> NBB4, when expressed in <i>E. coli.</i> and purified by his-tag, binds to the EtnP promoter (BBa_K1996000) as demonstrated by electrophoretic mobility shift assay (Figure 1). When bound by EtnR1, the EtnP DNA is less able to migrate through the agarose gel and therefore presents at a higher band size.
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| − | <img src="https://static.igem.org/mediawiki/2016/b/b0/T--Sydney_Australia--GelShiftFigure.png"> <br>
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| − | Figure 1. Electrophoretic mobility shift assay for EtnP DNA. The 250 bp region of EtnP was PCR amplified and 100 ng of DNA incubated with 6 mg of protein. The protein-DNA mixtures were run on a 3% agarose gel at 150 V for 100 minutes and stained with Gel Green overnight for visualisation. Lane 1: NEB 100 bp ladder. Lane 2: EtnP DNA only. Lane 3: EtnR1 protein only. Lane 4: EtnR1 protein and EtnP DNA. Lane 5: BSA protein and EtnP DNA.
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 01:38, 21 October 2016
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Applications of BBa_K1996002
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