Difference between revisions of "Part:BBa K1481002"
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This part generates a superfolder green fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose. | This part generates a superfolder green fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose. | ||
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Latest revision as of 20:33, 20 October 2016
sfGFP under arabinose promoter
This part generates a superfolder green fluorescent protein with his-tag for easy protein purification. It is controlled by a pBAD arabinose-inducible promoter. It also contains a coding sequence for the AraC protein, crucial for repression of pBAD in absence of arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1260