Difference between revisions of "Part:BBa K1983008:Design"

(→‎Design Notes)
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Original suffix: TACTAGTAGCGGCCGCTGCAG
 
Original suffix: TACTAGTAGCGGCCGCTGCAG
 
Part suffix: AACTAGTAGCGGCCGCTGCAG
 
Part suffix: AACTAGTAGCGGCCGCTGCAG
 +
 +
===Csm4 mutation list===
 +
 +
LA72F; LA236F; LA99F; YA143F; LA115F; LA219F; LA225F; LA191F; LA46F; YA258F; IA76F; YA114F; YA175F; LA235F; LA293F; LA25F; LA204F; YA234F; LA178F; LA106F; LA142F; LA20F; LA38F; IA7F; YA78F; IA193F; YA3F; IA84F; YA6F; LA41F; LA50F; YA252F; MA229F; LA198F; MA43F; LA103F; LA280F; IA128F; LA36F; LA5F; LA156F
  
 
===Source===
 
===Source===

Revision as of 18:43, 20 October 2016


Streptococcus thermofilus Csm4 phenylalanine mutant M27 with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 796
    Illegal XhoI site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed including Esp3I restriction site before the start codon (ATG) for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.

Note: Due to technical issues, the part's suffix in the backbone is changed by one FIRST letter (T to A). However, it does not remove or add any other restriction sites and does not change the function of the suffix. The purpose of this note is to alert false negative results during sequencing if the part is used in the future.

Original suffix: TACTAGTAGCGGCCGCTGCAG Part suffix: AACTAGTAGCGGCCGCTGCAG

Csm4 mutation list

LA72F; LA236F; LA99F; YA143F; LA115F; LA219F; LA225F; LA191F; LA46F; YA258F; IA76F; YA114F; YA175F; LA235F; LA293F; LA25F; LA204F; YA234F; LA178F; LA106F; LA142F; LA20F; LA38F; IA7F; YA78F; IA193F; YA3F; IA84F; YA6F; LA41F; LA50F; YA252F; MA229F; LA198F; MA43F; LA103F; LA280F; IA128F; LA36F; LA5F; LA156F

Source

BBa_K1983008

References