Difference between revisions of "Part:BBa K1906001"

Latest revision as of 18:18, 20 October 2016

LtrB Group II intron Front Segment

A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The Lactococcus lactis LtrB intron is a group II intron that can target the ligated-exon sequence of the L. lactis ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.

Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.

This part is the first segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrA.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1906001 short</partinfo>
-<p>A Group II intron is a retrohoming element that can mediate the insertion of itself to a certain location of DNA. The <i>Lactococcus lactis</i> LtrB intron  is a group II intron that  can target the ligated-exon sequence of the <i>L. lactis</i> ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.
+<p>A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The <i>Lactococcus lactis</i> LtrB intron  is a group II intron that  can target the ligated-exon sequence of the <i>L. lactis</i> ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.</p>
-[[File:XJTLU-intron-front.jpeg|200px|thumb|right|Figure 1. The strucure of wildtype Ll.LtrB Group II intron. The intron contains a ORF that encodes a protein referred as LtrA, which is reseponsible for the mature and intergratation of intron RNA into DNA molecules.]]
+[[File:XJTLU-intron-front.jpeg|200px|thumb|right|Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.]]
-This part is the fist segment of non-transcriptional RNA, from the begining of the RNA transcript to the start condon of LtrB.
+<br>
+This part is the first segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrA.
 ===Usage and Biology===