Difference between revisions of "Part:BBa K1906001"
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<p>A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The <i>Lactococcus lactis</i> LtrB intron  is a group II intron that  can target the ligated-exon sequence of the <i>L. lactis</i> ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.
 
<p>A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The <i>Lactococcus lactis</i> LtrB intron  is a group II intron that  can target the ligated-exon sequence of the <i>L. lactis</i> ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.
 
[[File:XJTLU-intron-front.jpeg|200px|thumb|right|Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.]]
 
[[File:XJTLU-intron-front.jpeg|200px|thumb|right|Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.]]
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This part is the fist segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrB.
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This part is the first segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrB.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:15, 20 October 2016


LtrB Group II intron Front Segment

A Group II intron is a retro-homing element that can mediate the insertion of itself to a certain location of DNA. The Lactococcus lactis LtrB intron is a group II intron that can target the ligated-exon sequence of the L. lactis ltrB gene. The general structure of wildtype LtrB Group II intron is showed in Figure 1.

Figure 1. The structure of wild-type Ll.LtrB Group II intron. The intron contains an ORF that encodes a protein referred as LtrA, which is responsible for the mature and integration of intron RNA into DNA molecules.

This part is the first segment of non-transcriptional RNA, from the beginning of the RNA transcript to the start codon of LtrB.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]