Difference between revisions of "Part:BBa K2097000"
Ianoverman (Talk | contribs) |
Ianoverman (Talk | contribs) |
||
| Line 10: | Line 10: | ||
https://static.igem.org/mediawiki/2016/a/ad/T--Austin_UTexas--pH_Dependent_Promoter.jpeg | https://static.igem.org/mediawiki/2016/a/ad/T--Austin_UTexas--pH_Dependent_Promoter.jpeg | ||
<html> | <html> | ||
| − | <p><b>Figure 1.</b></p> | + | <p><b>Figure 1. </b></p> |
</html> | </html> | ||
<div align="left"> | <div align="left"> | ||
| + | |||
| + | <br> | ||
<partinfo>BBa_K2097000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2097000 SequenceAndFeatures</partinfo> | ||
Revision as of 18:10, 20 October 2016
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.
CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor for the downstream gene, YGCP in this case. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so only the appropriate BioBrick prefix/suffix and CpxR binding site were required. Used BBa_K2097002 as the control series.
Usage and Biology
Figure 1.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]