Difference between revisions of "Part:BBa K2097000"

Revision as of 17:55, 20 October 2016 (view source) Ianoverman (Talk | contribs) ← Older edit		Revision as of 17:55, 20 October 2016 (view source) Ianoverman (Talk | contribs) Newer edit →
Line 16:		Line 16:
−	<!-- -->	+
	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
	<partinfo>BBa_K2097000 SequenceAndFeatures</partinfo>		<partinfo>BBa_K2097000 SequenceAndFeatures</partinfo>

---

Revision as of 17:55, 20 October 2016

CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor for the downstream gene, YGCP in this case. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so only the appropriate BioBrick prefix/suffix and CpxR binding site were required. Used BBa_K2097002 as the control series.

Usage and Biology