Difference between revisions of "Part:BBa K2082003"

 
 
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<partinfo>BBa_K2082003 short</partinfo>
 
<partinfo>BBa_K2082003 short</partinfo>
  
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Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein CDS
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Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein (CFP) CDS
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2082003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2082003 SequenceAndFeatures</partinfo>
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<html>
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This device consists of:
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<ul>
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<li>an Anderson promoter (BBa_J23106)</li>
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<li>a RBS (BBa_B0034)</li>
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<li>a GFP CDS</li>
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<li>a C-terminal split-CFP CDS</li>
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</ul>
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<br>
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This device can be used in combination with <a href="https://parts.igem.org/Part:BBa_K2082002">BBa_K2082002</a> to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.
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<figure class="figure">
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  <img src="https://static.igem.org/mediawiki/parts/e/ed/Bielefeld_CeBiTec_2016_10_20_bifc_gfp.png" class="figure-img" alt="" width=60%>
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  <figcaption class="figure-caption"><b><i>E. coli</i> with BBa_K2082003. Green fluorescence, caused by GFP.</b></figcaption>
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</figure>
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</html>
  
  

Latest revision as of 17:35, 20 October 2016


Split CFP Bimolecular fluorescence complementation (BiFC) with GFP

Anderson promoter, RBS, Fluorescent protein (GFP) CDS and Split fluorescent protein (CFP) CDS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 86
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a RBS (BBa_B0034)
  • a GFP CDS
  • a C-terminal split-CFP CDS

This device can be used in combination with BBa_K2082002 to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.
E. coli with BBa_K2082003. Green fluorescence, caused by GFP.