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TorA-BtuF

The TorA-BtuF Biobrick consists of a TorA signal sequence linked to BtuF, a protein capable of binding vitamin B₁₂. The TorA signal peptide allows export of fully-folded proteins through the inner membrane via the Tat (Twin-Arginine translocation) system. This construct thus enables export of vitamin B₁₂ bound to BtuF out of the cytoplasm. The TorA sequence codes for an amino-terminal signal peptide that harbours a twin-arginine motif which is vital for the recognition by the Tat system. The TorA signal sequence and the sequence coding for BtuF are connected by a linker of 15 bases, coding for the five amino acids following the signal peptide in trimethylamine-N-oxide reductase from E.coli.

Usage and Biology

BtuF

BtuF is the periplasmic binding protein for the vitamin B12 transporter BtuCD from E.coli (Cadieux et al., 2002). While some bacteria and archaea are capable of its synthesis, E.coli belongs to the majority of prokaryotes that contain transport systems to import B₁₂ (Warren et al., 2002). Its transmembrane transport is achieved by the Btu (B twelve uptake) system composed of BtuB, an outer membrane TonB-dependent transporter (Cadieux et al., 1999), and the ABC transporter BtuCDF, which is located in the inner membrane. While BtuC and BtuD compose respectively the trans-membrane domain and the ABC (Bassford et al., 1977), BtuF is the periplasmic binding protein. It has a size of 30.19 kDa and is composed of two globular domains, between which vitamin B₁₂ is bound, linked by a rigid interdomain α-helix (Karpowich et al., 2003). A crystal structure of the protein bound to B12 is available ([http://www.rcsb.org/pdb/explore/explore.do?structureId=1N4A PDB1N4A]; Karpowich et al., 2003).

The Tat export pathway and its signal peptide

In bacteria and archaea, proteins located outside the cytoplasm can reach their destination via either the Sec or the Tat (twin-arginine translocation) export pathway. While the Sec system translocates proteins in an unstructured state, the Tat apparatus has the unusual property of transporting fully folded proteins (Palmer and Berks, 2012). This system is very flexible in regard to the types of proteins that can be exported and the number of exported proteins highly differs between organisms. The E.coli Tat system is capable of transporting substrates up to 70 Å in diameter (Berks et al., 2000). Many exported proteins containing non-covalently bound cofactors use this pathway, because the cofactor is held in place by the protein folding. The Tat pathway is only used by proteins containing certain types of cofactors that are classified as metal-sulphur clusters or nucleotide based cofactors, which include among others also cobalamins (Berks et al., 2003).

Proteins are targeted to the Tat apparatus by amino-terminal signal peptides that are normally cleaved by an externally facing signal peptidase (Lüke et al., 2009). The cleavage is mediated by an AxA motif. The overall architecture is similar to Sec signal peptides and includes a tripartite structure with a basic n region at the N terminus, a hydrophobic h region in the middle and a polar c region at the C terminus. The key element of a Tat signal peptide is the highly conserved twin-arginine motif, defined as SRRXFLK (see figure 1). The two arginines are almost always invariant, while the other residues occur with a frequency of > 50 %. The amino acid at position X is usually polar (Palmer and Berks, 2012).

The TorA signal peptide used in this biobrick belongs to the trimethylamine-N-oxide reductase from E.coli, a well characterized protein exported via the Tat system. Using this signal peptide, it has already been achieved to export a heterologous protein normally transported by the Sec pathway through the Tat system (Christóbal et al., 1999).

Figure 1. Schematic view of the Tat signal peptide aligned with signal sequences from proteins exported via the tat pathway. Residues that match the Tat consensus are shown in red, with the twin arginines underlined. (modified from Palmer and Berks, 2012)

Sequence and Features

Functional Parameters

It could be shown that TorA-BtuF is expressed as well as translocated to the periplasm in Raoutella planticola and Shimwellia blattae.

Figure 2:

TorA-BtuF was expressed in S. blattae. After harvesting, the cells were fractionated. TorA-ButF, which has a calculated molecular weight of 37.22 kDa and contains a polyhistidine-tag, is clearly present in the periplasmic fraction (PP).

Figure 3:

TorA-BtuF was expressed in R. planticola. After harvesting, the cells were fractionated. TorA-ButF, which has a calculated molecular weight of 37.22 kDa and contains a polyhistidine-tag, is clearly present in the periplasmic fraction (PP).

Additionally, it was proven that the translocation ot TorA-BtuF to the periplasm led to the presence of higher amounts of vitamin B₁₂ in the periplasm of R. planticola and S. blattae compared to cells without TorA-BtuF expression.

Figure 3:

TorA-BtuF was expressed in R. planticola and S. blattae. After harvesting, the cells were fractionated. The periplasmic and cytoplasmic fractions were then submitted to a photometric B₁₂ assay.

[http://2016.igem.org/Team:Goettingen/Results Here] you can learn more about the experiments performed.

References

Bassford P. J., Jr., Kadner R. J. 1977 Genetic Analysis of Components Involved in Vitamin B12 Uptake in Escherichia coli. J. Bacteriol. 132:796–805.

Berks B. C., Sargent F., Palmer T. 2000 The Tat protein export pathway. Mol Microbiol. 35(2):260-74.

Berks, B. C., Palmer, T., Sargent, F. 2003 The Tat protein translocation pathway and its role in microbial physiology. Adv. Microb. Physiol. 47:187–254.

Cadieux N, Bradbeer C, Reeger-Schneider E, Köster W, Mohanty AK, Wiener MC, Kadner RJ. 2002 Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli. J Bacteriol. 184(3):706-17.

Cadieux N., Kadner R. J. 1999 Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter. Proc. Natl. Acad. Sci. U. S. A. 96:10673–10678.

Christόbal S., de Gier J.-W., Nielsen H. and von Heijne G., 1999 Competition between Sec- and Tat- dependent protein translocation in Escherichia coli. EMBO J. Vol. 18, No. 11: 2982-2990.

Karpowich N. K., Huang H. H., Smith P. C., Hunt J. F. 2003 Crystal Structures of the BtuF Periplasmic-binding Protein for Vitamin B12 Suggest a Functionally Important Reduction in Protein Mobility upon Ligand Binding. The Journal of Biological Chemistry 278, 8429-8434.

Lüke, I., Handford, J. I., Palmer, T. & Sargent, F. 2009 Proteolytic processing of Escherichia coli twin-arginine signal peptides by LepB. Arch. Microbiol. 191:919–925.

Palmer T., Berks B. C., 2012 The twin-arginine translocation (Tat) protein export pathway. Mature Reviews Microbiology 10:483-496.

Warren M. J., Raux E., Schubert H. L., Escalante-Semerena J. C. 2002 The biosynthesis of adenosylcobalamin (vitamin B12). Nat. Prod. Rep. 19:390–412.