Difference between revisions of "Part:BBa K1906004"
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This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-TS are essential for the production of Qbeta replicase holoenzyme.
 
This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-TS are essential for the production of Qbeta replicase holoenzyme.
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===Characterlization====
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===Characterlization===
 
[[File:20161019150339!SDS-TSTU-REP.jpg|200px|thumb|left|alt text]]
 
[[File:20161019150339!SDS-TSTU-REP.jpg|200px|thumb|left|alt text]]
  

Revision as of 17:10, 20 October 2016


Elongation Factor Tu

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-TS are essential for the production of Qbeta replicase holoenzyme.

Characterlization

alt text

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 280
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430