Difference between revisions of "Part:BBa K1980003"
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<partinfo>BBa_K1980003 short</partinfo>
 
<partinfo>BBa_K1980003 short</partinfo>
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<p>
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MymT is a small prokaryotic copper metallothein discovered in <i>Mycobacterium tuberculosis</i>. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a small flexible linker. This part has been codon optimised for E. coli.
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MymT is a small prokaryotic copper metallothein discovered in <i>Mycobacterium tuberculosis</i>. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a short hydrophilic, flexible linker. This part has been codon optimised for E. coli.</p>
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<br>
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<span class='h3bb'>Sequence and Features:</span>
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<partinfo>BBa_K1980003 SequenceAndFeatures</partinfo>
  
 
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===Usage and Biology===
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==Usage and Biology==
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<p> Our project aimed to detect and chelate dietary copper as a treatment for Wilson's Disease, a copper accumulation disorder.
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We decided that the ideal copper chelation protein would have these properties:
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</p>
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<ul class="textLiFont">
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<li>Should be able to bind multiple copper ions per peptide to increase the efficient use of cell resources. </li>
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<li>They should be from the prokaryotic domain because eukaryotic proteins can have expression issues in <i>Escherichia coli.</i> </li>
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</ul>
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<p>It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice.</p>
 
<p>It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice.</p>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1980003 SequenceAndFeatures</partinfo>
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Revision as of 12:55, 20 October 2016


MymT sfGFP

MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It can bind up to 7 copper ions but has a preference for 4-6. This version has a C terminal sfGFP with a C terminal hexahistidine tag for purification. The MymT and sfGFP are separated by a short hydrophilic, flexible linker. This part has been codon optimised for E. coli.


Sequence and Features:


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]