Difference between revisions of "Part:BBa K2070005"
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<partinfo>BBa_K2070005 short</partinfo> | <partinfo>BBa_K2070005 short</partinfo> | ||
− | This part was used to test the functionality of ECF sigma35 and its cognate promoter. | + | This part, consisting of a constitutive ECF sigma35 generator [https://parts.igem.org/Part:BBa_K2070013 BBa_K2070013] and ECF sigma35 inducible GFP reporter [https://parts.igem.org/Part:BBa_K2070004 BBa_K2070004] and was used to test the functionality of ECF sigma35 and its cognate promoter. |
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Revision as of 11:57, 20 October 2016
Constitutive ecf35 generator with ecf35 inducible GFP reporter
This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004 and was used to test the functionality of ECF sigma35 and its cognate promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 790
Illegal BsaI.rc site found at 1543