Difference between revisions of "Part:BBa K1955004"
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− | <b style="font-size:23px;">pSB1C3- | + | <b style="font-size:23px;">pSB1C3-Ovalbumin</b><br><br> |
The complete coding sequence of Ovalbumin (OVA) acquired from GenBank (Accession# AH002466.2) . OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen.<br><br> | The complete coding sequence of Ovalbumin (OVA) acquired from GenBank (Accession# AH002466.2) . OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen.<br><br> | ||
<b style="font-size:20px;">(1) The basic part checked by PCR: </b> | <b style="font-size:20px;">(1) The basic part checked by PCR: </b> |
Latest revision as of 08:50, 20 October 2016
pSB1C3-Ovalbumin
The complete coding sequence of Ovalbumin (OVA) acquired from GenBank (Accession# AH002466.2) . OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen.
(1) The basic part checked by PCR:
We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8% agarose gel by electrophoresis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 2108 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1235