Difference between revisions of "Part:BBa K2120403"

Revision as of 08:24, 20 October 2016

B0015+tetR+B0034+J23109+ptet+B0034+mazF+B0015

This is one of our application circuit.

Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.

We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.

Go our Parts Registry to learn more.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 851
Illegal NheI site found at 874
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2120403 short</partinfo>
+This is one of our application circuit.
+Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
+We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
+Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more.