Difference between revisions of "Part:BBa K2114002:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies.  
+
The cotZ gene comes from the genome of <i>B. subtilis</i>. The anti-GFP nanobody is derived camelid heavy-chain antibodies.  
  
 
===References===
 
===References===

Latest revision as of 06:28, 20 October 2016


aGFPnano_HA_G4S_cotZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of a HA epitope tag and a flexible GGGGS linker by primer extensions.


Source

The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies.

References