Difference between revisions of "Part:BBa K2066034:Design"

 
(→‎Design Notes)
 
(3 intermediate revisions by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
to be replaced
+
the part does have a SpeI cutsite in the Spinach aptamer. DTU-Denmark 2014 designed a Spinach aptamer with the cutsite removed. We’re going to use the original spinach to replicate the results of Pothoulakis et al. as exactly as possible and try to move to the Broccoli aptamer (which is RFC10 compatible) as soon as possible.
−
 
+
  
 +
UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"
  
 
===Source===
 
===Source===
  
−
to be replaced
+
This part was created using gBlock synthesis by IDT.
  
 
===References===
 
===References===

Latest revision as of 02:54, 20 October 2016


mCherry + Spinach, J23107 + B0034


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1052
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
    Illegal SpeI site found at 1052
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1052
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part does have a SpeI cutsite in the Spinach aptamer. DTU-Denmark 2014 designed a Spinach aptamer with the cutsite removed. We’re going to use the original spinach to replicate the results of Pothoulakis et al. as exactly as possible and try to move to the Broccoli aptamer (which is RFC10 compatible) as soon as possible.

UNS sequences from Torella et. al 2013 "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly"

Source

This part was created using gBlock synthesis by IDT.

References