Difference between revisions of "Part:BBa K2150016"

 
 
(6 intermediate revisions by 2 users not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2150015 short</partinfo>
+
<partinfo>BBa_K2150016 short</partinfo>
  
−
pT7 + RBS + GFP + D.TER
+
T7 promoter and RBS and GFP, with double terminator.  
−
 
+
−
<!-- Add more about the biology of this part here
+
 
===Usage and Biology===
 
===Usage and Biology===
 +
We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed a leakage of T7 promoter on high-copy plasmid pSB1C3.
 +
 +
[[File:pT7+GFP.png|350px]]
 +
 +
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K2150015 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2150016 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2150015 parameters</partinfo>
+
<partinfo>BBa_K2150016 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 00:31, 20 October 2016


pT7 GFP

T7 promoter and RBS and GFP, with double terminator.

Usage and Biology

We use this part to produce a high level of reporter protein GFP in our simulation system where T7 RNA polymerase is present. We observed a leakage of T7 promoter on high-copy plasmid pSB1C3.

PT7+GFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690