Difference between revisions of "Part:BBa J37015"

 
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<partinfo>BBa_J37015 short</partinfo>
 
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Prey Genotype + Reporter
 
Prey Genotype + Reporter
  
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===Usage and Biology===
 
===Usage and Biology===
  
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'''Biology:'''
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*In nature, quorum sensing is used for cell-cell communication to co-ordinate particular behaviours or actions once the cell population has reached a sufficiently large size.
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*This part generates the quorum sensing molecule, AHL via a positive feedback loop:
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::*The tetR promoter constitutively produces LuxR. AHL then forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI causes the production of AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
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*The positive feedback loop is triggered once cell population reaches a certain size.
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*The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].
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'''Usage:'''
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*This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule).
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'''Other Uses:'''
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*This part could be used to amplify a signal: (see experience page)
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::*A small amount of AHL present in the cell can be used to trigger the production of much more AHL via the positive feedback loop of this part.
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*This part could be used to monitor cell population size.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_J37015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J37015 SequenceAndFeatures</partinfo>

Latest revision as of 16:17, 20 August 2007

Prey Molecule Generator (AHL) + GFP Reporter

Prey Genotype + Reporter

Usage and Biology

Biology:

  • In nature, quorum sensing is used for cell-cell communication to co-ordinate particular behaviours or actions once the cell population has reached a sufficiently large size.
  • This part generates the quorum sensing molecule, AHL via a positive feedback loop:
  • The tetR promoter constitutively produces LuxR. AHL then forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI causes the production of AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
  • The positive feedback loop is triggered once cell population reaches a certain size.
  • The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].


Usage:

  • This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule).


Other Uses:

  • This part could be used to amplify a signal: (see experience page)
  • A small amount of AHL present in the cell can be used to trigger the production of much more AHL via the positive feedback loop of this part.
  • This part could be used to monitor cell population size.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2400