Difference between revisions of "Part:BBa J37015:Experience"

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This part was tested by the Brown iGEM team in August 2007. Some interesting and very repeatable results were obtained. It was expected that this part, J37015, would produce significantly more GFP than the part T9002, which differs only in that it has no LuxI gene and therefore no positive feedback loop. It was also expected that higher amounts of AHL would cause J37015 to produce more GFP than low amounts of AHL. Both of these hypotheses were proven false.
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The raw data for our experiments can be seen here: [[8-14-07_amplifier_test.xls]]
  
 
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Revision as of 15:45, 20 August 2007

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J37015

User Reviews

UNIQ1b21806ec75e02ec-partinfo-00000000-QINU UNIQ1b21806ec75e02ec-partinfo-00000001-QINU


This part was tested by the Brown iGEM team in August 2007. Some interesting and very repeatable results were obtained. It was expected that this part, J37015, would produce significantly more GFP than the part T9002, which differs only in that it has no LuxI gene and therefore no positive feedback loop. It was also expected that higher amounts of AHL would cause J37015 to produce more GFP than low amounts of AHL. Both of these hypotheses were proven false.

The raw data for our experiments can be seen here: 8-14-07_amplifier_test.xls

UNIQ1b21806ec75e02ec-partinfo-00000002-QINU

•••••

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006 IGEM:IMPERIAL/2006]

The [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols/J37015 protocol for J37015] was largely based on the [http://openwetware.org/wiki/Endy:F2620/Transfer_Curve/Protocols protocol by Drew Endy and Barry Canton] for the receiver BBa_F2620. Results and analysis for this part can be found [http://openwetware.org/wiki/IGEM:Imperial/Results/J37015 here].

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