Difference between revisions of "Part:BBa K1941004"

Latest revision as of 23:32, 19 October 2016

scCyc1_2PP7

Scaffold RNA with two PP7 hairpins that targets the region c6 of CYC1 promoter

Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpins are two times PP7, PP7 being a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 231
Illegal NgoMIV site found at 260
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1941004 short</partinfo>
-Scaffold RNA that targets the region c6 of CYC1 promoter and recruits two times the protein repressor module PCP-Mxi via two effector protein recruitments site PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and two times the PCP-binding RNA hairpin, PP7.
+Scaffold RNA with two PP7 hairpins that targets the region c6 of CYC1 promoter
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpins are two times PP7, PP7 being a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.
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