Difference between revisions of "Part:BBa B0031:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| + | |||
| + | ===Characterizations of BBa_B0031=== | ||
| + | The Alverno_Ca team characterized BBa_K2066036, which includes BBa_B0031, as well as a series of other plasmids using TX-TL, a mean of in vitro transcription and translation using cell extract. Below is a graph of normalized expression of BBa_K2066036 in comparison to the other palsmids in the sequence. | ||
| + | https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--W%26M_corrected.png | ||
| + | |||
| + | This graph shows the fluorescence of the plasmids, over a period of 12 and a half hours, in a plate reader. | ||
| + | https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--W%26M_plasmid_time_traces_in_TXTL%282%29.png" | ||
| + | |||
===Applications of BBa_B0031=== | ===Applications of BBa_B0031=== | ||
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| + | William and Mary iGEM 2016 | ||
| + | |||
| + | https://static.igem.org/mediawiki/2016/5/58/T--William_and_Mary--RBS_Characterization_Curves.png | ||
| + | |||
| + | We characterized the strengths of the Anderson RBS library. | ||
===User Reviews=== | ===User Reviews=== | ||
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<br><br> | <br><br> | ||
| − | See <a href="https://parts.igem.org/Part:BBa_B0034:Experience">Experience page of BBa_B0034</a>for more details. | + | See <a href="https://parts.igem.org/Part:BBa_B0034:Experience">Experience page of BBa_B0034</a> for more details. |
<br><br> | <br><br> | ||
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<td class='row'><b>Declared efficiency</b></td> | <td class='row'><b>Declared efficiency</b></td> | ||
</tr><tr> | </tr><tr> | ||
| − | <td class='row'> | + | <td class='row'>B0030</td><td class='row'>0.40</td><td class='row'>1.6814</td><td class='row'>2.45</td><td class='row'>0,6</td> |
</tr><tr> | </tr><tr> | ||
<td class='row'>B0031</td><td class='row'>0.01</td><td class='row'>ND</td><td class='row'>0.04</td><td class='row'>0,07</td> | <td class='row'>B0031</td><td class='row'>0.01</td><td class='row'>ND</td><td class='row'>0.04</td><td class='row'>0,07</td> | ||
| Line 85: | Line 99: | ||
<td class='row'><b>Declared efficiency</b></td> | <td class='row'><b>Declared efficiency</b></td> | ||
</tr><tr> | </tr><tr> | ||
| − | <td class='row'> | + | <td class='row'>B0030</td><td class='row'>1.72</td><td class='row'>0.96</td><td class='row'>0.45</td><td class='row'>0,6</td> |
</tr><tr> | </tr><tr> | ||
<td class='row'>B0031</td><td class='row'>0.03</td><td class='row'>0.78</td><td class='row'>0.028</td><td class='row'>0,07</td> | <td class='row'>B0031</td><td class='row'>0.03</td><td class='row'>0.78</td><td class='row'>0.028</td><td class='row'>0,07</td> | ||
Latest revision as of 23:32, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Characterizations of BBa_B0031
The Alverno_Ca team characterized BBa_K2066036, which includes BBa_B0031, as well as a series of other plasmids using TX-TL, a mean of in vitro transcription and translation using cell extract. Below is a graph of normalized expression of BBa_K2066036 in comparison to the other palsmids in the sequence.
This graph shows the fluorescence of the plasmids, over a period of 12 and a half hours, in a plate reader.
"
Applications of BBa_B0031
William and Mary iGEM 2016
We characterized the strengths of the Anderson RBS library.
User Reviews
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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UNIPV-Pavia iGEM 2011 |
NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.
Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA ( E. coli TOP10, high copy number plasmid) or LuxI:
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