Difference between revisions of "Part:BBa I763010"
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<partinfo>BBa_I763010 short</partinfo> | <partinfo>BBa_I763010 short</partinfo> | ||
| − | Promoter lambda - cI regulated - (R0051)regulates the expression of LacI reppression protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. | + | Promoter lambda - cI regulated - (R0051) regulates the expression of LacI reppression protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. |
This biobrick is necessary to repress pLac activity in absence of enough endogenous LacI. | This biobrick is necessary to repress pLac activity in absence of enough endogenous LacI. | ||
Revision as of 09:39, 20 August 2007
LacI repressor regulated by Plamda Promoter
Promoter lambda - cI regulated - (R0051) regulates the expression of LacI reppression protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. This biobrick is necessary to repress pLac activity in absence of enough endogenous LacI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1222
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]