Difference between revisions of "Part:BBa K1909009"

Latest revision as of 22:45, 19 October 2016

Weak Anderson / mTaz

BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).

Usage and Biology

The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.

Functional Parameters

This combination has the lowest expression of the three combinations BBa_K1909007, BBa_K1909008 and BBa_K1909009. Testing the combination showed that the cells grew very, very slow (for miniprep culture more than 48 hours, for colonies on LB-agar plates more than 72 hours).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 174

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 __NOTOC__
 <partinfo>BBa_K1909009 short</partinfo>
 BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6]  ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.